Same chapter3 marksDifficulty: Medium
What is gel electrophoresis and what is it used for?
Biotechnology: Principles and Processes
3 marksDifficulty: Medium
State three features a cloning vector must have.
Original Khojo Papers practice question — not from a past board paper.
Answer
It must have an origin of replication so that it can multiply inside the host. It must have a selectable marker, usually an antibiotic resistance gene, so that transformed cells can be identified. It must have unique restriction sites into which the foreign DNA can be inserted, and it should be small enough to be taken up easily.
No citable source has been recorded for this record. Treat it as practice material, not as fact.
Explanation
Insertional inactivation of a second marker gene is often used to identify recombinants.
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