Same chapter3 marksDifficulty: Medium
Describe the three steps of one cycle of the polymerase chain reaction.
Biotechnology: Principles and Processes
3 marksDifficulty: Medium
Name three ways in which foreign DNA can be introduced into a host cell.
Original Khojo Papers practice question — not from a past board paper.
Answer
Making the cell competent with divalent calcium ions and then giving a brief heat shock. Microinjection, in which the DNA is injected directly into the nucleus with a fine needle. Biolistics or the gene gun, in which the cells are bombarded with microscopic gold or tungsten particles coated with DNA. Disarmed pathogens such as Agrobacterium can also act as vectors.
No citable source has been recorded for this record. Treat it as practice material, not as fact.
Explanation
The method chosen depends on the type of cell.
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